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1 article(s) from Rublack, Nico

Synthesis of a bifunctional cytidine derivative and its conjugation to RNA for in vitro selection of a cytidine deaminase ribozyme

Nico Rublack and
Sabine Müller

Beilstein J. Org. Chem. 2014, 10, 1906–1913, doi:10.3762/bjoc.10.198

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Figure 1: Retrosynthetic analysis of the bifunctional cytidine derivative 1 for functionalization of a period...

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Figure 2: Introduction of the triazolyl moiety into the uridine derivative 7 generating synthon 3. I: 4 equiv...

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Figure 3: Preparation of synthon 4 and substitution of the triazolyl moiety of 3 to form the fully protected ...

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Figure 4: Synthesis of 2',3'-bis-O-(tert-butyldimethylsilyl)-1-[4-(N'-biotinyl-3,6-dioxaoctane-1,8-diamine)py...

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Figure 5: Formation of the phosphoramidite 2 from amino alcohol 13, and subsequent coupling to the 5'-O-depro...

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Figure 6: A) Reversed-phase HPLC purification of 1 after complete deprotection of 16. A represents the absorp...

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Figure 7: Reaction scheme of periodate oxidation of a 20mer model RNA followed by coupling of cytidine deriva...

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Figure 8: Reversed-phase HPLC analysis. A: Crude product of the coupling reaction between the 20mer model RNA...

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Published 15 Aug 2014

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